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Analytical Testing
Declaration of Purpose form
Analytical Testing
Resins Amino Acids & Derivatives Peptides
NITROGEN CONTENT
Substitution of amine resins and loaded resins

LOSS ON DRYING
Amount of residual solvent in resin

FTIR
Confirms Identity

PARTICLE SIZE
Measures size of bead

MESH SIZE
Bead size range and uniformity of beads

SWOLLEN VOLUME
Swelling of the resin in DCM solvent

DIPEPTIDE CONTENT
Test for dipeptide side products on loaded resins that may form in manufacture

CROSSLINKING
The level of linking between polymer chains. Relates to resin swelling

KAISER TEST
Test for unprotected Amine groups on resin


OPTIONAL TESTS
may attract an additional charge

ELEMENT ANALYSIS
Measures substitution

FmPip
Spectroscopic substitution measurement

MOLECULAR FORMULA
Empirical identity marker

MOLECULAR WEIGHT
Empirical identity marker

FTIR
Confirms identity

MELTING POINT
Indicates identity and purity

OPTICAL ROTATION
Indicates identity and purity

SOLUBILITY
Test for insoluble impurities, inorganic salts

NMR
Confirms identity

TLC
Test of purity

HPLC
Test of purity

NITROGEN ASSAY
Test of purity

RESIDUAL SOLVENTS
Residual solvents from manufacture. Purification by 1H NMR integration

LOSS ON DRYING
Test for residual volatile solvents

WATER CONTENT
Test hydrate form and residual water content


OPTIONAL TESTS
may attract an additional charge

OPTICAL PURITY
Measures amount of opposite enantiomer

KAISER TEST FOR FREE AMINO ACIDS
Test for unprotected amino acid impurities

ELEMENTAL ANALYSIS
Confirms molecular formula and purity
MOLECULAR FORMULA
Empirical identity marker

MOLECULAR WEIGHT
Empirical identity marker

SEQUENCE
Report of amino acid order

HPLC
Test purity

MASS SPEC
Confirms identity


OPTIONAL TESTS
may attract an additional charge

ENDOTOXIN
Level of pyrogenic substances

AMINO ACID ANALYSIS
Confirms identity

AMINO ACID CONTENT
Identifies amino acid

AMINO ACID SEQUENCE
Denotes order of amino acids

PEPTIDE CONTENT
Determines quanity of peptide in sample

CE
Test purity

WATER CONTENT
Indicates in part peptide content - mg of actual peptide in mg of product

ELLMAN’S TEST
Test for uncyclized cysteine side chains

NMR
Confirms identity

pH/pl Measurement
Confirms electronic state

Melting Point
Indicates identity and purity

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