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Analytical Testing
Declaration of Purpose form
Analytical Testing
Resins
Amino Acids & Derivatives
Peptides
NITROGEN CONTENT
Substitution of amine resins and loaded resins
LOSS ON DRYING
Amount of residual solvent in resin
FTIR
Confirms Identity
PARTICLE SIZE
Measures size of bead
MESH SIZE
Bead size range and uniformity of beads
SWOLLEN VOLUME
Swelling of the resin in DCM solvent
DIPEPTIDE CONTENT
Test for dipeptide side products on loaded resins that may form in manufacture
CROSSLINKING
The level of linking between polymer chains. Relates to resin swelling
KAISER TEST
Test for unprotected Amine groups on resin
OPTIONAL TESTS
may attract an additional charge
ELEMENT ANALYSIS
Measures substitution
FmPip
Spectroscopic substitution measurement
MOLECULAR FORMULA
Empirical identity marker
MOLECULAR WEIGHT
Empirical identity marker
FTIR
Confirms identity
MELTING POINT
Indicates identity and purity
OPTICAL ROTATION
Indicates identity and purity
SOLUBILITY
Test for insoluble impurities, inorganic salts
NMR
Confirms identity
TLC
Test of purity
HPLC
Test of purity
NITROGEN ASSAY
Test of purity
RESIDUAL SOLVENTS
Residual solvents from manufacture. Purification by 1H NMR integration
LOSS ON DRYING
Test for residual volatile solvents
WATER CONTENT
Test hydrate form and residual water content
OPTIONAL TESTS
may attract an additional charge
OPTICAL PURITY
Measures amount of opposite enantiomer
KAISER TEST FOR FREE AMINO ACIDS
Test for unprotected amino acid impurities
ELEMENTAL ANALYSIS
Confirms molecular formula and purity
MOLECULAR FORMULA
Empirical identity marker
MOLECULAR WEIGHT
Empirical identity marker
SEQUENCE
Report of amino acid order
HPLC
Test purity
MASS SPEC
Confirms identity
OPTIONAL TESTS
may attract an additional charge
ENDOTOXIN
Level of pyrogenic substances
AMINO ACID ANALYSIS
Confirms identity
AMINO ACID CONTENT
Identifies amino acid
AMINO ACID SEQUENCE
Denotes order of amino acids
PEPTIDE CONTENT
Determines quanity of peptide in sample
CE
Test purity
WATER CONTENT
Indicates in part peptide content - mg of actual peptide in mg of product
ELLMAN’S TEST
Test for uncyclized cysteine side chains
NMR
Confirms identity
pH/pl Measurement
Confirms electronic state
Melting Point
Indicates identity and purity
Tuesday 09 February, 2010
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